Plasmid

Part:BBa_K4790067:Design

Designed by: Yi Zheng   Group: iGEM23_NNU-CHINA   (2023-09-26)


PETase-node59


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5297
    Illegal EcoRI site found at 6114
    Illegal XbaI site found at 5163
    Illegal PstI site found at 1140
    Illegal PstI site found at 5415
    Illegal PstI site found at 5547
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5297
    Illegal EcoRI site found at 6114
    Illegal NheI site found at 6036
    Illegal PstI site found at 1140
    Illegal PstI site found at 5415
    Illegal PstI site found at 5547
    Illegal NotI site found at 6234
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5297
    Illegal EcoRI site found at 6114
    Illegal BglII site found at 5097
    Illegal BamHI site found at 5291
    Illegal XhoI site found at 6243
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5297
    Illegal EcoRI site found at 6114
    Illegal XbaI site found at 5163
    Illegal PstI site found at 1140
    Illegal PstI site found at 5415
    Illegal PstI site found at 5547
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5297
    Illegal EcoRI site found at 6114
    Illegal XbaI site found at 5163
    Illegal PstI site found at 1140
    Illegal PstI site found at 5415
    Illegal PstI site found at 5547
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3317
    Illegal NgoMIV site found at 3477
    Illegal NgoMIV site found at 5065
    Illegal NgoMIV site found at 5258
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1314
    Illegal SapI.rc site found at 2396


Design Notes

None.


Source

The original sequence of pET-22b was downloaded from NCBI. Node 59 was constructed through Ancestral Sequence Reconstruction (ASR).

References